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THE EXPRESSION OF MURINE Qa REGION GENE PRODUCT(S) IN L CELL TRANSFORMANTS
Author(s) -
Fernandez N.,
Festenstein H.,
Alonzo A.,
Biro P. A.,
Labeta M.,
Carolan E.,
Houlihan J.,
Mellor A.,
Flavell R.
Publication year - 1986
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1986.tb01127.x
Subject(s) - gene , biology , gene expression , gene product , product (mathematics) , genetics , cell , expression (computer science) , computational biology , microbiology and biotechnology , computer science , programming language , mathematics , geometry
SUMMARY The cosmid H3.5, containing genes mapping to the murine H‐2 Qa region, was used to transfect L cells by the calcium phosphate co‐precipitation method. The resultant transfected cells expressed a Qa‐like determinant as detected by an immune serum raised against the transfectant cells and Qa specific monoclonal antibodies. Two‐dimensional gel analysis revealed the expression of a class I‐like heavy chain with a similar molecular mass to the Qa2 antigens of the positive strain B10 and B10.A but with a different isoelectric point. The cosmid H3.5 spans 40 kb of DNA and contains at least one complete Qa region gene which encodes the Qa‐like determinant detected in this study.