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ELECTROPHORETIC RESOLUTION OF TWO RAT CLASS I ALLOANTIGENS EXPRESSED ON (WF x F344)F 1 LIVER CELLS IN PRIMARY CULTURE
Author(s) -
Hunt J. M.,
Buckley M. T.,
Laishes B. A.,
Butcher G. W.,
Desai P. A.
Publication year - 1986
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1986.tb01126.x
Subject(s) - primary culture , microbiology and biotechnology , resolution (logic) , electrophoresis , class (philosophy) , biology , primary (astronomy) , high resolution , chemistry , cell culture , immunology , physics , genetics , philosophy , computer science , astrophysics , remote sensing , epistemology , artificial intelligence , geology
SUMMARY Polyvalent alloantisera, prepared by reciprocal immunization of F344 ( RT1 lv1 haplotypes) and WF ( RT1 u haplotype) rats, as well as monoclonal antibodies, were used to immunoprecipitate class I alloantigens from detergent extracts of monolayer cultures of 35 S‐methionine‐labelled liver cells. Two‐dimensional IEF/SDS‐PAGE gel analysis resolved the RT1.A lv1 and RT1.A u class I antigens expressed on the liver cells in culture.