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MOLECULAR DEFINITION OF RETROVIRUS‐INDUCED ANTIGENS RECOGNIZED BY TUMOUR‐SPECIFIC H‐2‐RESTRICTED CYTOLYTIC T LYMPHOCYTES
Author(s) -
Plata F.,
LangladeDemoyen P.,
Abastado J.P.,
Berbar T.,
Kourilsky P.
Publication year - 1986
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1986.tb01110.x
Subject(s) - retrovirus , cytolysis , antigen , biology , virology , immunology , microbiology and biotechnology , genetics , cytotoxicity , virus , in vitro
SUMMARY The antigenic specificities of H‐2‐restricted, tumour‐specific cytolytic T lymphocytes (CTL) were studied at the molecular level using CTL from BALB.B and C57BL/6 ( H‐2 b ) mice sensitized to an H‐2 b Gross murine leukaemia virus (MuLV)‐induced tumour. Target cells were produced by the double trans‐fection of mouse L cells (H‐29 with the cloned H‐2K b or H‐2P gene and retro‐viral DNA derived from a molecular clone of Akv MuLV (closely related to Gross MuLV). Doubly transfected L cells which express either H‐2K b or H‐2D b antigen and retroviral antigens are lysed in a virus‐specific manner by Gross MuLV‐immune CTL. The existence of two independent Gross MuLV‐immune CTL subpopulations, one restricted by H‐2K b and the other by H‐2D b , is thus confirmed. Gross MuLV‐immune CTL from both BALB.B and C57BL/6 mice killed L cells that express Akv MuLV gag gene products and H‐2K b or H‐2D b antigen. In contrast, only CTL from C57BL/6 mice killed L cells that express Akv MuLV env gene products and H‐2K b or H‐2D b . This indicates that specific recognition of MuLV‐induced antigens by CTL can be selective and varies according to the origin of the CTL.