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PHENOTYPIC HETEROGENEITY OF HLA PRODUCTS ON HUMAN MELANOMA CELLS
Author(s) -
Parmiani G.,
Taramelli D.,
Anichini A.,
Andreola S.,
Cascinelli N.,
Fossati G.
Publication year - 1986
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1986.tb01106.x
Subject(s) - antigen , human leukocyte antigen , melanoma , monoclonal antibody , phenotype , biology , immunology , metastatic melanoma , pan t antigens , antibody , microbiology and biotechnology , cancer research , genetics , gene
SUMMARY The expression of class I and class II HLA antigens has been studied on primary and metastatic human melanomas, and on cell clones derived from the latter. A panel of monoclonal antibodies and flow cytofluorometric analysis were used to evaluate the presence of HLA‐A, B, C and ‐DR, DQ antigens on freshly isolated tumour cells. HLA class I antigens were present on 91% and 93% of primary and metastatic tumours, respectively. Sixty per cent of primary and 50% of metastatic melanomas expressed HLA‐DR antigens, whereas 38% and 21% of cases were positive for HLA‐DQ. A marked heterogeneity was evident among primary and metastatic lesions for expression of class I and II antigens. Similiar findings were obtained by analysing the phenotype of melanoma clones which indicates that a marked antigenic heterogeneity for class I and II HLA antigens occurs even among clones isolated from short‐term cultures of metastatic melanomas.