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REPRESSION OF CLASS I H‐2K,H‐2D ANTIGENS ON GR9 METHYLCHOLANTHRENE‐INDUCED TUMOUR CELL CLONES IS RELATED TO THE LEVEL OF DNA METHYLATION
Author(s) -
Bonal F. J.,
Pareja E.,
Martin J.,
Romero C.,
Garrido F.
Publication year - 1986
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1986.tb01099.x
Subject(s) - psychological repression , methylcholanthrene , dna methylation , dna , methylation , microbiology and biotechnology , class (philosophy) , biology , genetics , gene , philosophy , carcinogen , gene expression , epistemology
SUMMARY The correlation between gene expression and DNA methylation is well established. In the present study we examined DNA methylation as a potential regulatory mechanism of class I histocompatibility antigen expression. We selected for study two cell clones from the same mouse tumour (GR9). One of them is H‐2 class I‐positive (A7) and the other negative (B9). Using five enzymes and three different probes, no rearrangement or deletion was detected in the MHC class I genes of the negative clone compared with the positive one. These results do not provide an explanation for the differences observed in the expression of class I antigens in A7 (K d , D d positive) and B9 (K d , D d negative). We then studied the DNA methylation status in both clones by Southern blot analysis. Clear differences between the K d , D d positive and negative clones were detected. Furthermore, both K d and D d were clearly expressed after culturing the H‐2 class I negative clone with the demethylating agent 5‐azacytidine. These results suggest that the methylation of certain MHC cytoshes is a regulatory mechanism of H‐2 class I gene expression.