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STRUCTURAL VARIATIONS IN THE H‐2 GENES OF AKR LYMPHOMAS
Author(s) -
Hui K.,
Minamide L.,
Prandoni N.,
Festenstein H.,
Grosveld F. G.
Publication year - 1986
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1986.tb01092.x
Subject(s) - southern blot , biology , microbiology and biotechnology , genomic dna , gene , dna , locus (genetics) , restriction enzyme , genome , genetics
SUMMARY K36.16 is an AKR H‐2 k thymoma which expresses an aberrant H‐2D d ‐like allospecificity, does not have a detectable amount of the H‐2K k syngeneic antigen and grows very easily in syngeneic mice. By DNA‐mediated gene transfer experiments, we were able to obtain transformed clones which do express the H‐2K k molecules and are rejected by AKR mice. Southern hybridization was performed to assess whether any gross changes had occurred in the K36.16 H‐2K locus or elsewhere in the MHC, which might explain the lack of H‐2K expression and/or the presence of the aberrant H‐2D d ‐like allospecificity. Specific H‐2 class I DNA probes were used to compare the K36.16 genomic DNA with normal AKR thymus DNA after digestion with a variety of restriction enzymes. After hybridization with the pH‐2IIa probe a 2.8 kb ‘Hind III’ fragment was identified in the K36.16 genomic DNA which is absent from AKR DNA. The pH‐2IIa probe detects the third, transmembrane and cytoplasmic domains of class I genes. Although these changes are indicative of MHC genome modifications it is not yet possible to link these specific Southern blot pattern variations with the phenotypic changes mentioned above.