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PREFERENTIAL EXPRESSION OF CATFISH LIGHT CHAIN IMMUNOGLOBULIN ISOTYPES IN ANTI‐DINITROPHENYL ANTIBODIES
Author(s) -
Lobb C. J.
Publication year - 1986
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1986.tb01079.x
Subject(s) - ictalurus , catfish , immunoglobulin light chain , isotype , antibody , biology , dinitrophenyl , immune system , humoral immunity , microbiology and biotechnology , immunization , immunology , fish <actinopterygii> , monoclonal antibody , fishery
SUMMARY Previous studies indicated that immunoglobulins of the channel catfish, Ictalurus punctatus , contained two distinct isotypes of light chains. These different light chains, designated F and G, are reminiscent of κ and λ light chains of higher animals. This study was undertaken to establish whether or not there was preferential expression of one of the light chains in antibodies induced to the dinitrophenyl moiety during the course of the catfish humoral antibody response. The results indicated that, although antibody produced very early (1‐2 weeks) after primary immunization contained significant amounts (∼20%) of G light chains, the vast majority (∼90%) of antibody produced later (3 weeks through 1 year) was of the F isotype. Thus fish, as well as higher vertebrates, can express different isotypes of antibody during the immune response.