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LONG‐TERM PASSIVE ENHANCEMENT OF ALLOGENEIC SKIN GRAFTS WITH MONOCLONAL ANTIBODIES
Author(s) -
Hansen Ted H.,
Koprak Samuel L.,
Wormstall EvaMarie,
Olson Beverly J.,
Jackson Ronald D.
Publication year - 1985
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1985.tb00843.x
Subject(s) - term (time) , monoclonal antibody , medicine , antibody , monoclonal , immunology , physics , quantum mechanics
SUMMARY Monoclonal antibodies (mAb) to graft‐specific class I or class II major histocompatibility antigens were tested for their ability to enhance the survival of allogeneic skin transplants. Mutant mouse strains were grafted with wild type tissue to restrict the antigenic differences being recognized For allogeneic recognition of the class I antigen L d , mutant BALB/ c‐H‐2 dm2 (dm2) mice were grafted with wild type BALB/cKh skin, and two dm2 anti‐BALB/cKh mAb, 23‐10‐1 and 30‐5‐7, were tested for their ability to enhance. The anti‐L d antibody 23‐10‐1 (IgM) was found not to enhance the survival of BALB/c skin of dm2 mice. 30‐5‐7, however, an IgG 2a antibody of indistinguishable specificity from 23‐10‐1, prolonged graft survival for approximately 5 days. For recognition of selected Ia b determinants, mutant B6. C‐ H‐2 bm12 (bm12) mice were grafted with wild type B6/Kh skin, and mAb specific for the serological change(s) in bm12 were tested for their ability to enhance. The anti‐Ia b antibody 25‐9‐17 (IgG 2a ) was found not to enhance B6 grafts on bm 12 mice. However, the enhancement seen with 25‐9‐17 using (C3H x bm12)F 1 recipients was extraordinary, such that treated mice had a mean survival time three times that of the controls. Since 25‐9‐17 is of C3H origin, these results suggest that allotype (or possibly idiotype) compatibility is important in antibody enhancement. Another anti‐Ia b antibody 28‐16‐8 (IgM), also of C3H origin, failed to enhance a B6 graft on (C3H x bm 12)F 1 mice. These studies demonstrate, therefore, that mAb can be used to enhance skin grafts and that both isotype and allotype may be important parameters which influence the ability of an antibody to enhance.