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ANALYSIS OF A SEROLOGICAL DETERMINANT OF H‐Y ANTIGEN: EVIDENCE FOR CARBOHYDRATE SPECIFICITY USING AN H‐Y SPECIFIC MONOCLONAL ANTIBODY
Author(s) -
Shapiro Mark,
Goldberg Ellen H.
Publication year - 1984
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1984.tb01057.x
Subject(s) - monoclonal antibody , glycoconjugate , serology , antigen , antibody , microbiology and biotechnology , enzyme , chemistry , monoclonal , carbohydrate , biology , biochemistry , immunology
SUMMARY Using a monoclonal antibody to H‐Y antigen we characterized the molecular determinant responsible for H‐Y reactivity. H‐Y‐positive cells were treated with enzymes which alter carbohydrate structure and then were tested for their ability to absorb monoclonal anti‐H‐Y antibody. Indications are that the serological determinant recognized by this antibody is a glycoconjugate containing terminal non‐reducing and internal galactosyl residues.