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H‐2 CONTROL OF POLYADENYLATED mRNA PRODUCTION IN THE SPLEEN OF MICE INJECTED WITH BRUCELLA ANTIGENS
Author(s) -
Pierrez J.,
Guerci O.,
Oth D.
Publication year - 1984
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1984.tb01049.x
Subject(s) - spleen , messenger rna , splenocyte , stimulation , antigen , brucella abortus , microbiology and biotechnology , rna , biology , brucella , polyadenylation , lipopolysaccharide , chemistry , immunology , endocrinology , gene , genetics , brucellosis
SUMMARY RNA was extracted from the splenocytes of Brucella abortus antigen stimulated mice and of control mice. The proportion of chromatographically separated polyadenylated 11.2S mRNA, was determined. With the technique used, only stimulated mice exhibited significant amounts of this RNA species. The highest level was reached 1 day after the stimulation, and the decay from this level presented an oscillatory form during the 4 weeks following the injection. In two different genetic backgrounds, H‐2 b mice did not respond to the stimulus, in contrast to H‐2 a and H‐2 f mice. H‐2 b /H‐2 f heterozygotes behaved roughly as intermediate between H‐2 b and H‐2 f mice. This genetic control seems to parallel the genetic control of some Brucella ‐induced, thymus‐dependent events previously described.