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H ‐ 2 ANTIGENS OF CERTAIN t AND NON‐t MICE REACT WITH THE SAME MONOCLONAL ANTIBODY
Author(s) -
Balber A. E.,
Amos D. B.,
Artzt Karen
Publication year - 1984
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1984.tb01047.x
Subject(s) - epitope , spleen , microbiology and biotechnology , biology , monoclonal antibody , antigen , antibody , lymph node , immunoprecipitation , mutation , immunology , gene , genetics
SUMMARY Monoclonal antibody 212.i.4.2 mediated complement‐dependent lysis of spleen and lymph node cells carrying the t w1 , t w12 , t w71 , t 6 , t w73 , and t Lub1 haplotypes, while cells from mice carrying 11 other t haplotypes were not lysed. The antibody also detected an epitope controlled by genes in the H‐2D d region of non‐t mice. A molecule of 46,000 molecular weight was immunoprecipitated by 212.i.4.2 from detergent extracts of 125 I‐labelled spleen cells of +/t w12 and B10.D2 mice. The H‐2 dm2 mutation did not alter the expression of the epitope recognized by 212.i.4.2. However, the H‐2 dm1 mutation decreased the reactivity of lymphoid cells with the antibody in cytotoxicity tests, and 212.i.4.2 immunoprecipitated little or no protein from extracts of B10.D2(R106) spleen cells which carry the H‐2 dm1 mutation.