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AN INTERESTING MONOCLONAL ANTI‐N PRODUCED FOLLOWING IMMUNIZATION WITH HUMAN GROUP O, NN ERYTHROCYTES
Author(s) -
Fletcher A.,
Harbour C.
Publication year - 1984
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1984.tb01046.x
Subject(s) - monoclonal antibody , microbiology and biotechnology , papain , immunization , antibody , spleen , agglutination (biology) , monoclonal , neuraminidase , cell culture , trypsin , chemistry , biology , virology , immunology , biochemistry , virus , enzyme , genetics
SUMMARY Spleen cells from Balb/c mice given multiple injections of intact human erythrocytes (group O, NN) were fused with NS1 myeloma cells. Culture fluids from the resulting hybrid cells were screened for agglutinating antibody against a panel of erythrocytes. One cell line, 2/23, secreted an IgM antibody which reacted more strongly with NN than with MM cells. Neuraminidase or papain treatment of erythrocytes abolished agglutination whereas trypsin treatment did not. Reactions with U‐erythrocytes of different MN phenotypes confirmed the anti‐N specificity of monoclonal antibody 2/23. This is the first report of monoclonal anti‐N stimulated by the immunization of mice with intact erythrocytes.