Premium
ANTIGEN BINDING TO LYMPHOID CELLS FROM UNIMMUNIZED MICE
Author(s) -
DeLuca D.
Publication year - 1984
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1984.tb01039.x
Subject(s) - antigen , lymphatic system , immunology , biology , microbiology and biotechnology
SUMMARY In order to determine the specificity of antigen binding by double antigen‐binding lymphocytes obtained from mouse bone marrow and spleen, three types of experiments were performed (1) a high excess of unlabelled antigen was tested for its ability to inhibit the binding of unrelated antigen to both single and double antigen‐binding cells; (2) polyvalent anti‐mouse immunoglobulin was assessed for its ability to inhibit antigen binding; and (3) the ability of one antigen to co‐cap (or codistribute) with either another antigen or antiimmunoglobulin was studied to determine the spatial relationship of these components on the cell membrane. In order to study an adequate number of double ABC, these cells were enriched by using NIP‐specific ABC as a starting population. The data indicate that double antigen binding occurs via independent immunoglobulin cell surface receptors which can be spatially separated from one another under appropriate capping conditions.