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LACK OF H‐2L d LOCUS PRODUCTS ON A BALB/c FIBROSARCOMA EXPRESSING H‐2 k ‐LIKE ALIEN ANTIGENS
Author(s) -
Ballinari, D.,
Pierotti, M. A.,
Sensi M. Luisa,
Parmiani G.
Publication year - 1983
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1983.tb01024.x
Subject(s) - antigen , fibrosarcoma , microbiology and biotechnology , balb/c , biology , pan t antigens , neoplasm , cytotoxic t cell , ht1080 , serology , in vitro , histocompatibility , immune system , antibody , immunology , genetics , human leukocyte antigen
SUMMARY The presence of H‐2L d antigens was evaluated in methylcholanthrene‐induced BALB/c fibrosarcomas by a varity of approaches. Transplantation experiments showed that BALB/c‐H‐2 dm2 mice, a mutant strain whose cells do not express H‐2L d antigens, after immunization with BALB/c normal tissues developed a resistance to the growth of two tumours (C‐3 and GI‐17), but not to a third neoplasm, C‐1, which is known to have H‐2 d ‐as well as H‐2 k ‐like alien antigens. In vitro experiments with cytotoxic T lymphocytes generated against L d antigens confirmed a loss of L d antigens on C‐1 but not on C‐3 tumour cells. Serological experiments with an anti‐L d serum again revealed the presence of H‐2L d determinants on C‐3 but not on C‐1 cells. Biochemical analysis in SDS‐PAGE of immunoprecipitates obtained by specific anti‐H‐2 sera with NP40 lysates of the tumours studied could detect H‐2K d , H‐2D d and H‐2L d antigens in C‐3 fibrosarcoma cells whereas K d and D d were the only H‐2 d molecules found in C‐1 lysate along with the H‐2 k ‐like specificities. The possible genetic mechanisms which may explain this apparent gain and loss modification of the H‐2 profile of C‐1 are discussed.