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STUDIES OF CONGENITALLY IMMUNOLOGICALLY MUTANT NEW ZEALAND MICE: VIII. CELL‐SURFACE PHENOTYPE OF SPLEEN CELLS OF Xid AND NUDE MICE
Author(s) -
Skelly R. R.,
Bray K. R.,
Gershwin M. E.,
Dumont F.,
Ahmed A.
Publication year - 1983
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1983.tb00798.x
Subject(s) - congenic , spleen , biology , immune system , ratón , antibody , surface immunoglobulin , immunology , antigen , microbiology and biotechnology , phenotype , b cell , gene , genetics
SUMMARY Surface immunoglobulin on spleen cells from NZB and NZB/W mice and congenic mice bearing the nude or X‐linked immune defective ( X id ) gene was examined by flow microfluorometry with regard to both the frequency of positive cells and density expressed on the cell. These data indicate that although the frequency of unseparated sIg + B lymphocytes is equivalent among all of these groups of mice, the densities of sIgM and sIgD are different. Spleen cells from these mice were also separated by free‐flow electrophoresis and analyzed in a similar manner. This analysis demonstrated the absence of a subpopulation of B lymphocytes with a low electrophoretic mobility and low expression of sIgM. These studies suggest that maturational and/or activation states of the B cells in mice bearing the X id or nude genes are different from those seen in the parent strains of mice. Such alterations in cell‐surface antigens correlate with differences in the natural history of immunopathology of the autoimmune disease in these congenic colonies of New Zealand mice.