Premium
SUPRAMOLECULAR RELATIONSHIPS OF MEMBRANE ANTIGENS ON THE MURINE THYMOCYTE
Author(s) -
Lynes M. A.,
Karl Margaret,
DiBiase Kathleen,
Flaherty Lorraine
Publication year - 1982
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1982.tb01009.x
Subject(s) - thymocyte , paraformaldehyde , heterologous , antigen , incubation , monoclonal antibody , chemistry , antibody , cell , microbiology and biotechnology , biology , biophysics , immunology , biochemistry , organic chemistry , gene , cd8
SUMMARY The topographical relationship of Thy‐1, Ly‐1, Ly‐2, and T200 were examined on the murine thymocyte. The inhibition of the binding of radiolabelled monoclonal antibody after incubation with unlabelled heterologous antibody was used as a measure of the proximity of the target antigens on the cell surface. On unfixed cells, prior incubation with anti‐Thy‐1.2 impeded the attachment of labelled anti‐Ly‐1, anti‐Ly‐2, and anti‐T200. Anti‐T200, but not anti‐Ly‐1, or anti‐Ly‐2, was capable of impeding the attachment of labelled anti‐Thy‐1.2 on unfixed cells. In general, gentle fixation with paraformaldehyde did not alter these inter‐relationships. The possible functional contributions of such supramolecular relationships are discussed.