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LY ‐ AND ECOTROPIC MuLV ANTIGENS ARE SEPARTE ENTITIES
Author(s) -
Langdon W. Y.,
McKenzie I. F. C.,
Shellam G.R.
Publication year - 1982
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1982.tb01004.x
Subject(s) - antigen , antibody , antiserum , virology , biology , virus , titer , rickettsia , microbiology and biotechnology , immunology
SUMMARY Murine alloantisera to lymphocyte (Ly) alloantigens also contain antibodies to murine leukaemia viruses (MuLV) in high titre. A number of such sera were extensively absorbed with ecotropic AKR murine leukaemia virus to remove contaqiminating anti‐MuLV antibodies. The virus‐absorbed and unabsorbed antisera wre then examined by the radioimmune precipitation (RIP) assay for anti‐MuLV antibodies, and for anti‐Ly antibodies by cytotoxicity assays against specific target cells. The results presented here demonstrate that whilst virus absorption markdely reduced the anti‐viraql titres of the alloantisera they ahd no effect on the anti‐Ly titres of any of them. This indicated that none of the Ly antigens which was examined cross reacts with the antigens of ecotropic AKR‐MuLV and further suggest that Ly antigens are not encoded by the genes of endogenous ecotropic MuLVs. It was also demonstrated that the majority of the constaninating anti‐MuLV antibodies present in anti‐Ly‐6.2 proteins of the virus.