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MOUSE MONOCLONAL ANTI‐N: II. PHYSICOCHEMICAL CHARACTERIZATION AND ASSESSMENT FOR ROUTINE BLOOD GROUPING
Author(s) -
Fraser R. H.,
Munro A. C.,
Williamson A. R.,
Barrie E. K.,
Hamilton E. A.,
Mitchell R.
Publication year - 1982
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1982.tb00986.x
Subject(s) - polyclonal antibodies , monoclonal antibody , antiserum , reagent , monoclonal , chemistry , antibody , microbiology and biotechnology , blood grouping , immunology , biology , organic chemistry , abo blood group system
SUMMARY The precise physicochemical conditions under which two examples of mouse monoclonal anti‐N can be used as blood grouping reagents have been defined. The antibodies were shown to belong to the IgG1 and IgG2b subclasses respectively and both reacted within discrete ranges of temperature and pH with optimal reactions occurring at 20d̀C or less and pH 8.5. Under these conditions and at concentrations of 2‐3 μg/ml, the antibodies were used, in parallel with conventional polyclonal antisera, to type a series of random blood donors. The results obtained with one of the monoclonal antibodies and the polyclonal reagents were in perfect agreement and the monoclonal antibody was judged to have considerable potential as an N‐grouping reagent.