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THE DETECTION OF PUBLIC Ia ANTIGENS ON THE SURFACE OF B6‐C. H‐2 b m 1 2 BLAST CELLS
Author(s) -
Michaelides Mary,
McKenzie I. F. C.
Publication year - 1982
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1982.tb00978.x
Subject(s) - antigen , microbiology and biotechnology , chemistry , biology , immunology
SUMMARY The B6‐C. H‐2 bm12 (bm 12 ) strain has previously been described as a spontaneously occurring mutant wherein the parental C57BL/6 Ia b specificities (Ia.3, 8, 9, 15 and 20) are absent or reduced in amount. To further characterize the bm 12 mutant the antigenic phenotype of LPS‐activated bm 12 blast cells was determined with monoclonal and conventional anti‐Ia antibodies. By direct testing and absorption the public Ia.8, 9 and 15 specificities were readily detected on both parental C57BL/6 and mutant bm 12 blast cells, and the amounts of these antigens on activated bm 12 cells were similar to or less than those found on C57BL/6 blast cells. The findings demonstrate that the Ia‐1 mutation has not resulted in an absolute loss of these specificities, but has altered their expression, or their accessibility to antibody on resting cells. By contrast, the private Ia. 20 specificity and another specificity (designated Ia b ) could not be detected on bm 12 blast cells.