Premium
EQUIVALENT DECREASE OF H‐2Kk AND H‐2Dk EXPRESSION AFTER VACCINIA VIRUS INFECTION
Author(s) -
Lakdhar M.,
Senik Anna
Publication year - 1982
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1982.tb00971.x
Subject(s) - biology , virus , vaccinia , multiplicity of infection , immunofluorescence , virology , serology , microbiology and biotechnology , cytolysis , in vitro , antibody , immunology , gene , recombinant dna , biochemistry , cytotoxic t cell
SUMMARY Expression of H‐2K k and H‐2D k molecules was studied by indirect immunofluorescence on vaccinia virus‐infected L s cells (derived from the L929 cell line), using D‐23 b and ASA‐21 monospecific alloantisera, respectively directed against the H‐2.23 and H‐2.32 private specificities of the H‐2 k haplotype. Our study demonstrates that the increase in vaccinia virus‐induced antigens on the membrane of infected cells (as a function of the multiplicity of infection) is associated with a concomitant decrease in the expression of both H‐2K k and H‐2D k serologically defined private specificities. Absorption experiments of D‐23 b and ASA‐21 sera, using infected or uninfected L s cells, also indicate that after virus infection, H‐2K k and H‐2D k private specificities are equally altered in their serological expression. We finally show that this alteration consists in a mere quantitative decrease of H‐2 molecules, since the cytolytic capacity of a rabbit anti‐H‐2 serum, specifically reacting against the constant part of the heavy chains of H‐2 molecules, was significantly more absorbed by uninfected cells than by infected cells. We conclude that no preferential decrease in the expression of H‐2 K k and H‐2 D k molecules is induced during cell infection by vaccinia virus.