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THE IMMUNE RESPONSE OF ALLOPHENIC MICE TO THE SYNTHETIC POLYMER GLØ *: III. ALLOTYPE ANALYSIS OF ANTI‐GLØ ANTIBODY
Author(s) -
Gorcyca D. E.,
McIvorNayeri Judith L.,
Maurer P. H.,
Warner Carol M.
Publication year - 1982
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1982.tb00967.x
Subject(s) - allotype , immune system , antibody , strain (injury) , microbiology and biotechnology , antibody response , c57bl/6 , biology , chemistry , immunology , anatomy
SUMMARY Allophenic mice were produced by aggregating 8‐cell embryos from a low responder (CBA) and a high responder (DBA/1) strain to the synthetic amino acid polymer GLø. In some strains of mice, the immune response to this polymer is controlled by two genes, α and β, which map to the IE/C and IA subregions of the H‐2 complex, respectively. The CBA strain is α+β ‐ whereas the DBA/1 strain can be considered to be α+β+. These two strains also differ in immunoglobulin allotype: CBA is allotype a and DBA/1 is allotype c . Eight CBA DBA/1 allophenic mice were immunized with GLø. Four of the mice gave a high secondary response to the polymer. The alloptype of the angibody from these mice was determined by affinity chromatography, and it was found that only allotype c was present. This means that all of the antibody was produced by high responder DBA/1 cells. Thus, there is no evidence for the cooperation of histoincompatible cells in the mounting of an immune response by allophenic mice.