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MONOCLONAL ANTI‐TYPE 2 H: AN ANTIBODY DETECTING A PRECURSOR OF THE A AND B BLOOD GROUP ANTIGENS
Author(s) -
Knowles R. W.,
Bai Y.,
Daniels G. L.,
Watkins Winifred
Publication year - 1982
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1982.tb00965.x
Subject(s) - abo blood group system , ulex europaeus , monoclonal antibody , biology , microbiology and biotechnology , lectin , antigen , antibody , glycoprotein , h antigen , blood type (non human) , agglutination (biology) , hemagglutination , biochemistry , immunology , agglutinin
SUMMARY A monoclonal antibody was produced which binds specifically to Type 2 H antigen of the ABO blood group system. The antibody, H11, is an IgM molecule which reacts by direct agglutination of red cells with the same pattern as other anti‐H reagents such as Ulex europaeus lectin. The specificity was determined by inhibition and adsorption with chemically defined oligosaccharides. H11 also reacted with poly(glycosyl)ceramide purified from group O red cells and glycoprotein H purified from human stomach mucosa and meconium. H11 differs from Ulex lectin, however, in that it was not inhibited by saliva from ABH secretor individuals or by glycoprotein H purified from human ovarian cysts or submaxillary glands.