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DIFFERENTIAL SUSCEPTIBILITY OF SPLEEN CELLS FROM C3H/HeN (H‐2k) AND C3HfB/HeN (H‐2kv1) MOUSE STRAINS TO CYTOTOXICITY BY MONOCLONAL ANTI‐Kk ANTIBODY (CLONE 11‐4)
Author(s) -
Martin W. J.,
Imamura M.
Publication year - 1981
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1981.tb01074.x
Subject(s) - spleen , clone (java method) , monoclonal antibody , antiserum , lysis , microbiology and biotechnology , antibody , biology , titer , monoclonal , cytotoxicity , virology , immunology , biochemistry , in vitro , gene
SUMMARY Spleen cells from C3HfB/HeN mice (H‐2 kv1 ) were markedly less susceptible to lysis by monoclonal anti‐K k antibody (Clone 11‐4) than spleen cells from C3H/HeN mice (H‐2 k ). Less than 50% of C3HfB/HeN cells were lysed even at the highest antibody concentration tested compared to 70% of C3H/HeN cells. The difference in end point titre of the antibody tested on cells from the two strains was 5‐10‐fold. Similar differences were not observed using the conventional H‐2K k ‐typing antiserum (D‐23).