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DEFINITION OF ALIEN H‐2 DETERMINANTS ON A FRIEND LEUKAEMIA BY ANALYSIS OF ALLOREACTIVITY OF CTL FROM PRIMARY MLC
Author(s) -
Greenberg P. D.,
Cheever M. A.,
Fefer A.
Publication year - 1981
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1981.tb00958.x
Subject(s) - ctl* , antigen , cytotoxic t cell , biology , recombinant dna , microbiology and biotechnology , virology , immunology , in vitro , gene , genetics , cd8
SUMMARY A Friend virus‐induced tumour of BALB/c (H‐2 d ) origin, HFL/d, was examined for the expression of alien H‐2 antigens. The alloantigens on HFL/d were typed by generating CTL in primary MLC with HFL/d as stimulator and measuring reactivity to targets with known H‐2 antigens, and confirmed by assessing recognition of HFL/d targets by CTL generated in primary MLC with stimulators expressing known H‐2 antigens. Potential cross‐reactivities between alloantigens were analysed by cold‐target inhibition experiments. BALB/c cells stimulated with HFL/d lysed H‐2 b targets, and BALB/c anti‐H‐2 b CTL lysed HFL/d; analysis with recombinant haplotypes demonstrated both H‐2K b and H‐2D b alien antigens on HFL/d. C57BL/6 (H‐2 b ) cells stimulated with HFL/d recognized H‐2K d , H‐2D d , and an additional determinant unique to HFL/d. (BALB/c x B6)F 1 cells also recognised a unique HFL/d determinant not of H‐2 b or H‐2 d origin. These unique determinants, which induced a strong cytotoxic response in primary MLC, were not shared by BALB/c or B6 tumours induced by cross‐reactive FMR viruses. Thus, HFL/d expressed the K and D antigens of its strain of origin, two typed alien H‐2 antigens, and at least one other untyped antigen which may represent an additional H‐2 determinant. These studies further demonstrate the utility of examining the reactivity of CTL generated in primary MLC to probe for the presence of alien H‐2 antigens.