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INCOMPATIBILITY FOR OR PRE‐IMMUNIZATION AGAINST M1s DETERMINANTS DECREASES LETHAL GRAFT‐ VERSUS ‐HOST REACTION DEVELOPED ACROSS NON ‐ H ‐ 2 AND/OR H‐2 BARRIERS
Author(s) -
HallePannenko* Olga,
Festenstein Hilliard
Publication year - 1981
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1981.tb00951.x
Subject(s) - phenotype , biology , spleen , microbiology and biotechnology , immunology , genetics , gene
SUMMARY The effect of incompatibility for Mls determinants was studied in lethal graft‐ versus ‐host reaction (GVHR) in the mouse. GVHR was induced in adult recipients of the following H‐2 k strains: (AKR x B10.BR)F1 (Mls B /Mls b ); (C3H x B10.BR)F1 (Mls c /Mls b ); (CBA/J x B10.BR)F1 (Mls d /Mls b ) and (CBA/H x B10.BR)F1 (Mls b ). Recipient mice were heavily irradiated and grafted with bone marrow and spleen cells from H‐2 compatible B10.BR (H‐2 k , Mls b ) or H‐2 incompatible B10.D2 and B10 donors were normal, while those from B10.BR donors were either normal or pre‐immunized against the recipient strains. In all experiments the survival of recipients with Mls a /Mls b and Mls d /Mls b phenotypes, and only in one experiment of those with Mls c /Mls b phenotype was greater and/or the survival time longer than that of recipients expressing only Mls b . However, late deaths (> 120 days post grafting) observed after grafting of normal B10.BR cells were more frequent in Mls d /Mls b than in Mls b strains. On the other hand, when B10.BR donor cells were pre‐immunized against H‐2 k compatible (AKR x B10.BR)F1 (Mls a /Mls b ) or (CBA/J x B10.BR)F1 (Mls d /Mls b ) strains, the survival time of H‐2 incompatible (B10 x B10.BR)F1 (H‐2 b/k , Mls b ) recipients was longer than when donor cells were pre‐immunized against (CBA/H x B10.BR)F1 (Mls b ) strain. We conclude that donor incompatibility for Mls a or Mls d or donor‐pre‐immunization against Mls a or Mls d exerts a protective effect on lethal GVHR developed across non‐H‐2 or H‐2 barriers; the protective effect of Mls c is less efficient or absent. The Mls‐induced protective effect shows the following properties: (a) efficiency in vivo correlates with the capacity of the corresponding alleles to stimulate an in vitro MLR; (b) is efficient in either primary or secondary response to other minor antigens; (c) is not H‐2 restricted; (d) is nonspecific; (e) disappears late after grafting; (f) with respect to the genetic background, the early protective effect is followed, late after grafting, by an opposite effect which increases the mortality, suggesting that M/s locus determinants are capable of activating several cell populations with different biological functions.