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A MARMOSET T‐LYMPHOCYTE PROTEIN RELATED TO DEFINED HUMAN SERUM IMMUNOGLOBULIN AND FRAGMENTS
Author(s) -
Marchalonis J. J.,
Wang A. C.
Publication year - 1981
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1981.tb00754.x
Subject(s) - immunoglobulin light chain , antibody , microbiology and biotechnology , immunoprecipitation , isotype , biology , antiserum , antigen , radioimmunoassay , monoclonal antibody , chemistry , biochemistry , immunology
Summary Current concepts of the antigen‐specific receptor of thymus‐derived (T) lymphocytes suggest that this molecule bears immunoglobulin (Ig) variable (V) regions, but does not represent a classical serum antibody. We immunized rabbits with a globulin fraction obtained from an in vitro cultured marmoset T cell lymphoma line which expressed surface components related to immunoglobulins. Quantitative immunoprecipitation and competition radioimmunoassay studies carried out using characterized monoclonal human Igs and their chains and fragments show that this rabbit antiserum, raised aginst a T cell product, reacts with a particular, conformational V H ‐determinant which is formed by interaction with λ light chains and with two μ chain (IgM) related determinants. Neither μ chain determinant is a major isotype specific marker, and each occurs only on certain distinct subsets of serum μ chains. One is located in the Fc fragment; the location of the other μ related determinant is not known. These results show that this rabbit antiserum recognizes both variable and constant region determinants on the T cell receptor heavy chain. Rabbit antibody to the T cell product which cross‐reacts with serum μ chain was isolated by immune affinity chromatography on Sepharose‐derivatized covalently with one particular reactive IgM protein, the Waldenstrom macroglobulin Can (kμ). This antibody precipitated a biosynthetically‐labelled component of approximate M. W. 70,000 as assessed by polyacrylamide gel electrophoresis in Na Dod SO 4 ‐containing buffers. These data support the conclusion that certain T cells express and synthesize a molecule related to Ig V H ‐regions which also has a constant region sharing antigenic markers with IgM subpopulations.