Premium
CELLULAR AND MOLECULAR HETEROGENEITY OF H‐2K k ANTIGENS
Author(s) -
Eskinazi D. P.,
Molinaro G. A.,
Reisfeld R. A.,
Ferrone S.
Publication year - 1981
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1981.tb00747.x
Subject(s) - computational biology , biology
Summary Up to 70% of mouse spleen cells expressing H‐2K k alloantigens reacted with the monoclonal anti‐H‐2K k antibody 11‐4.1 in an indirect rosetting assay. When the cells that rosetted with the monoclonal antibody 11‐4.1 were removed by density centrifugation, the residual unreactive cells reacted with the polyclonal anti‐H‐2K k alloantiserum D23 but not with the monoclonal antibody 11‐4.1 indicating the existence of two cell populations. In sequential immunoprecipitation experimens, the glycoprotein fraction of NP40 extracts from H‐2K k cells, after removal of antigens reacting with the monoclonal antibody 11‐4.1 still contained structures reactive with the alloantiserum. Therefore, there are at least two antigenically distinct H‐2K k molecules which are differentially expressed on two subpopulations of spleen cells.