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GENETICS OF CHINESE HAMSTER MIXED LYMPHOCYTE REACTIVITY
Author(s) -
Jong B. de,
Anders G. J. P. A.,
Van De Meer I. H.,
Zijlstra J.,
Wierda J.
Publication year - 1980
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1980.tb00935.x
Subject(s) - chinese hamster , biology , lymphocyte , mixed lymphocyte reaction , allele , mitomycin c , hamster , phenotype , reactivity (psychology) , immunology , microbiology and biotechnology , genetics , peripheral blood , in vitro , immune system , pathology , t cell , gene , medicine , alternative medicine
Summary Two‐way and one‐way mixed lymphocyte cultures (MLC) with Chinese Hamster peripheral blood lymphocytes were performed. Reduction of the culture surface and addition of 2‐mercaptoethanol to the culture medium gives a marked enhancement of the reactivity of low numbers of lymphocytes in the MLC. Genetic analysis of the mixed lymphocyte reactivity (MLR) in the animals studied so far indicates that three or four different alleles are involved. Howerver, not all phenotypes could be traced. The samples of different stocks of Chinese Hamsters in Europe and the United States did not all share the same alleles and the samples were also different as regards the relative frequency of phenotypes. For DNA inhibition in stimulating cells mitomycin C treatment is less suitable than irradiation. Skin graft survival time was longer in animals which showed no MLR than in animals with a positive MLR.