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Fab 2 FRAGMENTS FROM HLA XENOANTISERA SPECIFICALLY BLOCK CYTOLYSIS MEDIATED BY HLA‐A, B ALLOANTISERA
Author(s) -
Belvedere Mariaclara,
Richiardi Patricia,
Pellegrino MicheleA.,
Ferrone S.
Publication year - 1980
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1980.tb00931.x
Subject(s) - cytolysis , allotype , antiserum , antibody , lytic cycle , human leukocyte antigen , biology , antigen , beta 2 microglobulin , microbiology and biotechnology , epitope , immunology , virology , virus , genetics , cytotoxicity , in vitro
Summary Fab 2 fragments from antisera raised in rabbits with partially purified cellular and serum HLA antigens were tested for their ability to block the cytolytic activity of operationally specific HLA‐A, B alloantisera. One Fab 2 fragment preparation blocked the cytolytic activity of all the HLA‐A,B alloantisera tested; the remaining nine inhibited the lytic activity of alloantisera to certain HLA‐A,B allospecificities, suggesting that these xenoantisera contain antibody to certain HLA‐A,B allotype determinants or to closely associated structures. In contrast to previous reports in the literature none of the xenoantisera contained significant amounts of antibodies to human β 2 ‐microglobulin.