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GENETIC REGULATION OF THE EXPRESSION OF H‐2K.5 ANTIGEN ON ERYTHROID CELLS BY H‐2K END
Author(s) -
Shiroishi T.,
Moriwaki K.
Publication year - 1980
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1980.tb00726.x
Subject(s) - congenic , antigen , biology , recombinant dna , microbiology and biotechnology , regulator , isoantigens , phenotype , immunology , genetics , gene
Summary Difference in the amounts of H‐2K.5 antigens present on erythrocytes was observed, using quantitative absorption method, among several B10 congenic strains. However, no such variation was seen on the lymphocytes and lung cells. The variability in the amount of this antigen on the erythrocyte surface was primarily dependent on the haplotype of the H‐2K end in the B10 recombinant strains examined. This suggests that the regulator of H‐2 expression on erythrocytes is in this region. Further genetic analysis confirmed that this regulation functions in the cis ‐position. Finally, the H‐2K.5 antigenic activity was expressed on the reticulocytes of all strains tested, but appeared lower in the type 2 group indicating that the regulation begins early in erythropoietic differentiation and eventually results in a complete loss of detectable activity.