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PUBLIC H‐2 SPECIFICITIES ARE TARGET DETERMINANTS FOR ALLOREACTIVE CYTOTOXIC T LYMPHOCYTES
Author(s) -
Vazquez A.,
Senik Anna,
Fridman W. H.,
NeauportSautes Catherine
Publication year - 1980
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1980.tb00713.x
Subject(s) - ctl* , cytotoxic t cell , biology , antiserum , antigen , microbiology and biotechnology , immunology , genetics , in vitro
Summary The specificity of he corss‐killing exerted by cytotoxic T lymphocytes (CTL's) generated against H‐2 region products was investigated in a 51 Cr release assay on a panel of target cells from a number of different H‐2 haplotypes. Their pattern of reaction shows that: (1) The target cells, expressing public specificities (H‐2.28, H‐2.1, H‐2.5, H‐2.3 or H‐2.8) which should, theoretically, be recognized by the CTL's were killed, while those expressing no public specificities, recognized according to the H‐2 chart by the CTL's wer not killed. (2) The CTL's generated against the H‐2.28 specificity expressed on the D region products cross react with target cells expressing this specificity in their K region products and vice versa. The same phenomenon was observed with the H‐2.1 specificity. These results provide evidence that public specificities are targets for CTL's. Antiserum reacting against the public specificity recognized by the CTL's was found to block the cross‐killing, however, to the same extent as antisera directed against any specificity recognized by the CTL's was found to block the cross‐killing, however, to the same extent as antisera directed against any specificity (private or public) expressed on the same molecule as the target determinant. Finally both the inhibition studies using anti‐H‐2 antisera and the direct cytotoxic assays showed that the public specificities of the H‐2.28 family carried by the H‐2.D and H‐2.L molecules were recognized by different subpopulations of CTL's.