Premium
EXPRESSION OF FOREIGN H‐2‐LIKE ANTIGENS BY A CHEMICALLY‐INDUCED MURINE TUMOUR (MCG4)
Author(s) -
Schirrmacher V.,
Garrido F.,
GarciaOlivares F.,
Perez M.,
Torres M. Dolores
Publication year - 1980
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1980.tb00707.x
Subject(s) - antigen , monoclonal antibody , antiserum , microbiology and biotechnology , serology , biology , typing , cytotoxicity , antibody , methylcholanthrene , virology , chemistry , in vitro , immunology , genetics , carcinogen
Summary Alien H‐2 k ‐like antigens were found to be expressed by a methylcholanthrene induced tumour of BALB/c (H‐2 d ) origin. H‐2 specificities of the k haplotype were detected on this tumour by a variety of serological techiques, including 51 Cr‐release cytotoxicity, microradioassay and absorption. The antisera employed were conventional polyspecific alloantisera, typing sera with restricted specificty and monoclonal hybridoma‐derived anti‐H‐2 k antibodies. The tumour has a low expression of the private specificty 31, which characterizes K d molecules, and does not seem to express the private specificities of D d , K k and D k molecules. It appears to express predominantly alien H‐2‐like antigens which are very similar to but not identical with normal H‐2 k molecules.