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H ‐ Y ANTIGEN: GENETIC CONTROL OF THE EXPRESSION AS DETECTED BY HOST ‐ VERSUS ‐ GRAFT POPLITEAL LYMPH NODE ENLARGEMENT ASSAY MAPS BETWEEN THE T AND H ‐ 2 COMPLEXES
Author(s) -
Králová Jarmila,
Lengerová Alena
Publication year - 1979
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1979.tb00697.x
Subject(s) - biology , microbiology and biotechnology , antigen , gene , allele , lymph node , haplotype , gene expression , genetics , immunology
Summary We are using the terms ‘low’ and ‘high’ expression when the H‐Y antigen on thymocytes of mouse parental strains is, respectively, weakly and strongly immunogenic for F 1 hybrids in a host‐versus‐graft popliteal lymph node enlargement assay. Using this assay we attempted to locate the genetic factor(s) responsible for the control of H‐Y expression within chromosome 17 since our previous study indicated linkage with the H‐2 complex. We produced to this aim recombinants from (B10.T x C3H/Di)F 1 hybrids where crossing over took place between H‐2 and T complexes. In this way we were able to locate a gene denoted Hye (for H‐Y expression) whose ‘high’ and ‘low’ alleles originated, respectively, from the B10 and C3H/Di strains. In the recombinants, the expression did not follow the H‐2 haplotype, but obviously depended on the position of crossing over between H‐2 and T. This pointed to the Hye gene mapping proximally from the H‐2 complex.