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DISCRIMINATION OF ANTIBODIES TO HEPATITIS B SURFACE ANTIGEN FROM ANTIBODIES TO INHERITED SERUM PROTEIN VARIANTS IN IMMUNE SERA OF HUMAN AND ANIMAL ORIGIN
Author(s) -
Solaas Marit Hornberg
Publication year - 1979
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1979.tb00687.x
Subject(s) - antigen , antibody , antiserum , precipitin , immune system , microbiology and biotechnology , biology , hepatitis , hepatitis b , virology , immunology , chemistry
Summary Antisera to hepatitis B surface (HB s ) antigen, both of human and rabbit origin, have been examined. The anti‐HB s serum derived from a multiply transfused patient did, in addition, contain antibodies directed against the inherited β‐lipoprotein antigen Ag(x), whereas one of the rabbit immune sera also contained antibody to the inherited Lp(a) antigen. Thus, in human, as well as in animal anti‐HB s sera, antibodies to inherited normal serum antigens may cause false positive reactions. This problem may be overcome by absorption procedures if appropriate control systems are avialable. False positive reactions caused by the Ag(x) antigen may also be avoided by use of agarose as supporting medium for the test, as Ag(x) precipitin lines do not appear in this medium. An undialysable high molecular weight component may be obtained from Oxoid ‘Ionagar’ by washing. When this added to agarose the Ag(x) antigen reaction appears also in this medium.