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THE IMMUNOGLOBULIN‐LIKET‐CELL RECEPTOR: I. IN SITU DEMONSTRATION OF IMMUNOGLOBULIN Fab‐REGION DETERMINANTS OF RODENT T‐ AND B‐LYMPHOCYTES USING CHICKEN ANTIBODIES *
Author(s) -
Marchalonis J. J.,
Warr G. W.,
Bucana C.,
Hoyer L. C.
Publication year - 1979
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1979.tb00685.x
Subject(s) - antibody , biology , microbiology and biotechnology , idiotype , surface immunoglobulin , receptor , immune system , antigen , immunoglobulin g , affinity chromatography , myeloma protein , immunoglobulin fc fragments , b cell , immunology , monoclonal antibody , biochemistry , enzyme
Summary Antibodies were raised in chickens to the (Fab') 2 fragment of normal murine IgG and to the κ‐myeloma protein MOPC 41. Following appropriate absorptions or purification by immune affinity chromatography the chicken antibodies bound specifically to both T‐ and B‐cells of mice, rats and guinea‐pigs as detected by quantitative cytofluorescence, radioactive binding assays and transmission and scanning immunoelectronmicroscopy. These antibodies are directed against polypeptide determinants of the Fab fragment, block antigen binding by purified idiotype‐bearing murine antibodies, and provide useful probes for visualization and analysis of immunoglobulin‐like surface receptors of rodent B‐ and T‐cells.