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ABSENCE OF FOUR H‐2 d ANTIGENIC SPECIFICITIES IN AN H‐2 d SARCOMA
Author(s) -
Garrido F.,
Perez M.,
Torres M. Dolores
Publication year - 1979
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1979.tb00333.x
Subject(s) - antigen , microbiology and biotechnology , sarcoma , gene , ascites , tumor associated antigen , cancer research , biology , chemistry , antibody , pathology , immunology , biochemistry , medicine , monoclonal antibody
Summary MCG4 is a BALB/c sarcoma induced as a solid tumour with 0.2 mgs of methylcholantrene. The primary tumour was serially transplanted subcutaneously in syngeneic mice. The ascites form obtained was used to study the expression of H‐2 antigeneic specificities in a postlabelling radioassay. MCG4 did not express H‐2D.4 (private specificity of H‐2 d haplotypes) as well as H‐2.3, H‐2.8 and H‐2.13 (public specificities). In addition it expressed H‐2.5 (a public specificity not present in H‐2 d cells). These results were confirmed by quantitative absorption analysis using MCG4 and positive‐negative normal lymphoid cells for a particular specificity. Results are discussed with regard to the control of expression of H‐2 antigens by regulatory genes.