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GENE EXPRESSION OF A REGION OF CHROMOSOME 17 DURING MURINE SPERMATOGENESIS
Author(s) -
Erickson R. P.
Publication year - 1977
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1977.tb00918.x
Subject(s) - spermatogenesis , biology , somatic cell , antigen , phosphoglycerate kinase , microbiology and biotechnology , sperm , gene , antiserum , genetics , endocrinology
SUMMARY The presence of H‐2‐linked gene products on spermatozoa and their time of appearance during spermatogenesis was determined. Thymus leukaemia antigen specificities 1, 2 and 3 could not be detected on spermatozoa by absorption of the antisera. Immunofluorescent studies with anti‐Sip sera did not reveal any specific reactivity with target spermatozoa. In contrast, H‐2D antigens were present on somatic as well as germ line components in testes so the time of their first appearance during spermatogenesis could not be precisely specified. Cell separation experiments indicate that H‐2D antigens are present on pachytene spermatocytes and increased in quantity on spermatids. The sperm‐specific isoenzyme of phosphoglycerate kinase, Pgk ‐2, appears at a later stage of spermatogenesis than do the H‐2 region antigens.