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THE DEFECT OF M k ERYTHROCYTES AS REVEALED BY SODIUM DODECYLSULPHATE‐POLYACRYLAMIDE GEL ELECTROPHORESIS*
Author(s) -
Dahr W.,
Uhlenbruck G.,
Knott H.
Publication year - 1977
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1977.tb00901.x
Subject(s) - sodium , chemistry , polyacrylamide gel electrophoresis , gel electrophoresis , electrophoresis , chromatography , gel electrophoresis of proteins , biochemistry , enzyme , organic chemistry
SUMMARY The membrane glycoprotein defect of M k red cells was studied using discontinuous sodium dodecylsulphate—polyacrylamide gel electrophoresis, carbohydrate analytical procedures and lectins. The results suggest that M k erythrocyte membranes contain only about half of the normal amount of the MN and Ss sialoglycoproteins, both molecules being not qualitatively different from normal. The major membrane protein exhibits an increased molecular weight in the above red cells. Some data on M g and Miltenberger class five cells are presented for comparison. The results on M k erythrocytes support the operon concept of the MNSs blood group system proposed previously.