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SOME IMMUNOCHEMICAL PROPERTIES OF Le a ‐AND Le b ‐ACTIVE SUBSTANCES IN HUMAN URINE
Author(s) -
Shinohara T.,
Yamamoto S.,
Iseki S.
Publication year - 1977
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1977.tb00898.x
Subject(s) - sephadex , size exclusion chromatography , chemistry , chromatography , urine , hemagglutination , fraction (chemistry) , gel permeation chromatography , biochemistry , enzyme , antibody , biology , organic chemistry , immunology , polymer
SUMMARY Le a ‐ and Le b ‐active substances present in normal human urine were isolated by sequential membrane filtration, column chromatography with DEAE‐Sephadex (A‐25), and gel filtration of Bio‐Gel (P‐6). Le a ‐active substance with the highest activity was obtained in the P‐1 fraction (0.8 mg/1) from urine of a non‐secretor type by the final gel filtration. Le b ‐active substance with the highest activity was also obtained in the P‐1 fraction (0.2 mg/1) from urine of a secretor type. The P‐1 of the non‐secretor inhibited Le a ‐anti‐Le a haemagglutination minimally at 4 μg/ml, and the P‐1 of the secretor inhibited Le b ‐anti‐Le b haemagglutination at 1 μg/ml. Both P‐1 fractions possessing Le a or Le b activity were characteristic in having a much higher fucose content than other isolated fractions, and seemed to be complex carbohydrates with molecular weights of the order of 4–5 × 10 3 .