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THE AVIDITY OF IgM ANTIBODY IN HIGH AND LOW RESPONDER RATS
Author(s) -
Davis B. K.,
Shonnard J. W.,
Gill T. J.
Publication year - 1977
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1977.tb00623.x
Subject(s) - avidity , antibody , antigen , immunization , bovine serum albumin , chemistry , immunoglobulin m , microbiology and biotechnology , immunology , strain (injury) , albumin , immunoglobulin g , biology , biochemistry , medicine
SUMMARY This study examined IgM antibody produced by highly responding ACI and poorly responding F344 rats following immunization with poly(Glu 52 Lys 33 Tyr 15 ) or poly(Glu 32 Lys 33 Tyr 15 ) aggregated with methylated bovine serum albumin (MeBSA). The ACI rats produced both IgM and IgG plaque‐forming cells (PFC) following immunization with either form of antigen. The F344 rats did not respond to unaggregated poly(Glu 52 Lys 33 Tyr 15 ), but they produced significant amounts of IgG PFC and extremely small amounts of IgM PFC after immunization with poly(Glu 52 Lys 33 Tyr 15 )/MeBSA. Both high and low responder rats had similar kinetic profiles of IgM antibody production, and this antibody had nearly identical avidity in both strains with no evidence for any maturation in avidity. thus, one of the genetic defects in the antibody response to poly(Glu 52 Lys 33 Tyr 15 ) is an inability of the F344 strain to produce large amounts of IgM in response to this antigen.