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MULTIPLE FOREIGN NON‐H‐2 DETERMINANTS ON THE SURFACE OF A CHEMICALLY‐INDUCED MURINE SARCOMA *
Author(s) -
Invernizzi G.,
Carbone G.,
Meschini Angela,
Parmiani G.
Publication year - 1977
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1977.tb00619.x
Subject(s) - cytotoxic t cell , biology , antigen , lymph node , serology , microbiology and biotechnology , in vitro , histocompatibility , major histocompatibility complex , c57bl/6 , lymph , antibody , immunology , pathology , biochemistry , human leukocyte antigen , medicine
SUMMARY By immunizing BALB/c (H‐2 d mice against normal tissues from C57BL/6J (H‐2 b ), C3Hf (H‐2 k ) and DBA/2 (H‐2 d ), but not from AKR (H‐2 k ) strains, resistance was induced to the subsequent challenge of the ‘syngeneic’ methyl‐cholanthrene‐induced BALB/c sarcoma ST5; lymph node cells from allo‐immune BALB/c mice were also able to exert a parallel cytotoxic effect against in vitro cultured ST5 cells. The involvement of foreign H‐2 specificities in the observed cross‐reactions was ruled out by absorptions of H‐2 monospecific sera and by the interallelic combinations used, thus suggesting that non‐H‐2 histocompatibility antigens were responsible for the above findings. By using the indirect isotopic antiglobulin assay, BALB/c anti‐C57BL/6J and anti‐C3Hf polyspecific sera were found to bind specifically to cultured ST5 cells. C57BL/6J and C3Hf, but not DBA/2, lymph node cells were able to absorb the anti‐ST5 activity of the anti‐C57BL/6J serum. These results indicated that ST5 cells expressed on their surface at least two different sets of foreign non‐H‐2 antigens: one shared by C57BL/6J and C3Hf tissues, and detected by both cell‐mediated and serological techniques; the other one belonging to DBA/2 tissues, and revealed mainly at the cell‐mediated level.