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GENETIC STUDIES IN INBRED RATS VIII. THE PRODUCTION OF XENOANTISERA AGAINST Ag‐B HISTOCOMPATIBILITY ANTIGENS
Author(s) -
Edwards P. A.,
Kunz H. W.,
Gill T. J.
Publication year - 1977
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1977.tb00614.x
Subject(s) - hyperimmunization , biology , antiserum , antigen , histocompatibility , antibody , cytotoxic t cell , immunization , immunology , microbiology and biotechnology , genetics , in vitro , human leukocyte antigen
SUMMARY Rabbit anti‐rat xenoantisera were raised by intravenous injection of splenic lymphocytes. After absorption with the appropriate rat red blood cells for removal of ‘species‐specific’ antibodies, many agglutinating specificities remained which were quite similar to the corresponding alloantisera with the exception of the Ag‐B5 haplotype. In attempts to produce specific Ag‐B xenoantisera, two groups of histocompatibility antigens were discovered: one consisting of Ag‐B1, Ag‐B3, Ag‐B4, Ag‐B7 and Ag‐B8 to which specific Ag‐B antisera could be produced, and another comprised of Ag‐B2, Ag‐B5 and Ag‐B6 to which specific Ag‐B antisera could not be made by any of several methods. Xenoantisera produced by a rapid hyperimmunization technique and all alloantisera tested were cytotoxic, whereas no cytotoxic antibodies could be detected in the xenoantisera produced by a longer course of immunization.