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STUDIES ON THE MEMBRANE GLYCOPROTEIN DEFECT OF En(a‐) ERYTHROCYTES
Author(s) -
Dahr W.,
Uhlenbruck G.,
Wagstaff W.,
Leikola J.
Publication year - 1976
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1976.tb00599.x
Subject(s) - glycoprotein , biology , erythrocyte membrane , microbiology and biotechnology , antigen , membrane glycoproteins , sodium , polyacrylamide gel electrophoresis , serology , membrane , biochemistry , immunology , chemistry , antibody , organic chemistry , enzyme
SUMMARY En(a‐) and En a En red cells from different sources were studied using biochemical and serological methods. The results suggest that Finnish En(a–) erythrocytes contain only ‘N’ but no M antigenic properties. Data on the members of the English En(a‐) family suggest that English En(a‐) red cells exhibit a combination of three rare alterations. The English En(a–) individuals are apparently heterozygous for the defects En and M k . In addition, the ‘N’ receptor on the Ss glycoprotein is, to all appearances, converted to a M (‘M’) antigen. These extraordinary data are brought to light by investigations on the children of the propositus (G.P. and J.P.), whose red cells are M‐N+S‐s+‘N’+‘M’‐ and M‐N+S+s+‘N’+‘M’+ respectively. Sodium dodecylsulphate polyacrylamide gel electrophoretic results indicate that the MN glycoprotein content is decreased by about 50 % in all English S+s+ En a En red cells, the Ss glycoprotein being normal. G.P. erythrocytes, however, have only about half of the normal MN and Ss glycoprotein content.