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ENZYMATIC CONVERSION OF HUMAN GROUP O RED CELLS INTO GROUP B ACTIVE CELLS BY α‐D‐GALACTOSYLTRANSFERASES OF SERA AND SALIVAS FROM GROUP B AND ITS VARIANT TYPES
Author(s) -
Kogure T.,
Furukawa K.
Publication year - 1976
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1976.tb00567.x
Subject(s) - group b , group a , saliva , titer , biology , enzyme , antigen , microbiology and biotechnology , galactosyltransferase , antibody , biochemistry , immunology , medicine
SUMMARY Salivas from group B secretor or non‐secretor, acting on O red cells in the presence of UDP‐galactose, each converted them into B active cells, which were agglutinated against anti‐B human serum (1:512) at the titre of thirty‐two‐fold, while secretor or non‐secretor group AB salivas converted O red cells into B active cells, which were agglutinated by anti‐B human serum (1:512) at the titre of eight‐ to sixteen‐fold. The results indicate that the α‐galactosyltransferases which participate in the biosynthesis of group B substance are secreted in group B or AB salivas of both secretor and non‐secretor types as well as in their sera. Agglutinabilities of enzymatically converted B‐active red cells against anti‐B human serum indicate that α‐galactosyltransferase activities of both serum and saliva from a weak B (B w ) individual, who has weak B antigens in red cells and saliva, were lower than those of normal group B. The α‐galactosyltransferase activities in group B m sera were lower than those of normal group B, while the enzyme activities in salivas of group B m were demonstrated to the same degree in normal group B salivas.