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A MOUSE PLASMA SUBSTANCE CARRYING β 2 ‐MICROGLOBULIN ACTIVITY AND LACKING IN H‐2 ALLOANTIGENIC ACTIVITY *
Author(s) -
Natori T.,
Tanigaki N.,
Pressman D.
Publication year - 1976
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1976.tb00563.x
Subject(s) - papain , beta 2 microglobulin , chemistry , substance p , microbiology and biotechnology , blood proteins , molecular mass , blood plasma , biochemistry , biology , enzyme , immunology , neuropeptide , receptor
SUMMARY A mouse plasma substance carrying β 2 ‐microglobulin activity and lacking in H‐2 alloantigenic activity was separated from the blood plasma of A/J strain female mice. The plasma substance had a molecular size of about 300,000–400,000 daltons and an electrophoretic mobility of α‐globulin. The plasma substance was split by papain digestion to a fragment of about 50,000–60,000 daltons that still carried β 2 ‐microglobulin activity. The papain‐split plasma substance was devoid of H‐2 alloantigenic activities and Thy‐1 and TL alloantigenic activities as well, but yet had a two‐component structure that was similar to papainsolubilized H‐2 molecules. It contained a 37,000‐dalton component linked non‐covalently to an 11,000‐dalton component, i.e. mouse β 2 ‐microglobulin. This plasma substance appears to be different from Ss protein or Slp protein, both found in mouse serum.