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CHROMOSOMAL ASSIGNMENT OF THE HL‐A COMMON ANTIGENIC DETERMINANTS IN MAN‐MOUSE SOMATIC CELL HYBRIDS *
Author(s) -
Smith Moyra,
Gold P.,
Shuster J.,
Tanigaki N.,
Pressman D.
Publication year - 1976
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1976.tb00561.x
Subject(s) - biology , gene , somatic cell , genetics , karyotype , chromosome , microbiology and biotechnology , somatic fusion , coding region
SUMMARY In the study presented here, man‐mouse somatic cell hybrid clones were examined by means of radioimmunoassays for the presence of both β 2 ‐microglobulin (β 2 m) and the HL‐A xenoantigenic determinant. In addition, the clones were examined for their karyotype and the expression of enzymes with known chromosomal assignments. The results obtained indicate that the gene coding for the HL‐A xenoantigenic determinant is carred on chromosome 6. The data obtained provides a direct demonstration that the gene coding for β 2 m segregates independently of that coding for the alloantigenic polypeptide chain of the HL‐A molecule, and that the gene coding for β 2 m is carried on chromosome 15.