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A RADIOASSAY FOR MACROPHAGES AND ITS APPLICATION FOR TESTING MACROPHAGE MEMBRANE ANTIGENS
Author(s) -
PeñaMartinez J.,
Schirrmacher V.,
Garrido F.,
Festenstein H.
Publication year - 1975
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1975.tb00548.x
Subject(s) - macrophage , antigen , uridine , cell , in vitro , trypsin , microbiology and biotechnology , phagocytosis , antibody , chemistry , rna , stimulation , biology , biochemistry , immunology , gene , enzyme , neuroscience
SUMMARY Monolayers of mouse peritoneal macrophages actively incorporated 14 C‐labelled uridine, an RNA precursor. This uptake did not seem to be due to in vitro activation or stimulation of the cells but rather to their high RNA metabolic activity. 14 C‐uridine uptake by the unstimulated adherent cells was a characteristic property of macrophages, because it was not reduced when the cells were (i) pretreated with trypsin (to detach remaining lymphocytes), (ii) precultured for several days (to allow PMN to die off) and (iii) pretreated with anti‐mouse immunoglobulin or anti‐θ serum plus complement. Pretreatment of macrophage monolayers with antibody plus complement, followed by a measurement of their 14 C‐uridine uptake could be used to test for anti‐macrophage activity, for instance in certain anti‐B cell or anti‐T cell sera, or to detect cell surface antigens on macrophages. With this technique we demonstrated the presence of H‐2 and Ia alloantigens on peritoneal macrophages. The presence of Ia antigens on macrophages may be of particular biological significance.