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SDS‐POLYACRYLAMIDE GEL ELECTROPHORETIC ANALYSIS OF THE MEMBRANE GLYCOPROTEINS FROM S‐s‐U‐ERYTHROCYTES
Author(s) -
Dahr W.,
Uhlenbruck G.,
Issitt P. D.,
Allen F. H.
Publication year - 1975
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1975.tb00529.x
Subject(s) - staining , electrophoresis , polyacrylamide gel electrophoresis , microbiology and biotechnology , glycoprotein , gel electrophoresis , red blood cell , membrane glycoproteins , silver stain , membrane , polyacrylamide , chemistry , biology , biochemistry , enzyme , genetics
SUMMARY It is shown that S‐s‐U‐erythrocytes have no detectable PAS‐3, B‐ and C‐glyco‐protein bands which are demonstrable by SDS‐polyacrylamide gel electrophoresis of red cell membranes and subsequent PAS‐staining. PAS‐1 and ‐2 are normal with respect to electrophoretic mobility and staining intensity. The staining intensity of PAS‐3, B and C from heterozygous defective erythrocytes is about half of normal. One S‐s‐U+ blood sample did not differ from the S‐s‐U‐bloods.