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GENETIC STUDIES IN INBRED RATS. IV. XENOANTISERA AGAINST RAT ERYTHROCYTE (Ag‐C) ANTIGENS *
Author(s) -
Poloskey Paul E.,
Kunz Heinz W.,
Gill Thomas J.,
Shonnard John W.,
Hansen Carl T.,
Dixon Barbara D.
Publication year - 1975
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1975.tb00521.x
Subject(s) - antiserum , antigen , antibody , microbiology and biotechnology , biology , lymphocyte , reagent , chemistry , immunology
SUMMARY Antibodies to the erythrocyte Ag‐C antigens were raised by immunizing New Zealand white rabbits with rat red cells. Evidence that Ag‐C antigens are not on lymphocytes includes: (1) Ag‐C could not be detected on rat peripheral, splenic or thymic lymphocytes by lymphocytotoxicity using anti‐Ag‐C antisera, (2) xeno‐antisera prepared against rat lymphocytes from which Ag‐B antibodies were absorbed did not agglutinate rat red cells, and (3) absorption of reagent Ag‐C antisera with lymphocytes did not remove the anti‐Ag‐C activity. Platelets also failed to absorb Ag‐C antibodies from reagent anti‐Ag‐C antisera. The mixed lymphocyte reaction between strains within the same major histocompatibility (Ag‐B) group but differing in Ag‐C type showed no stimulation. This finding corroborates the studies which failed to demonstrate Ag‐C on lymphocytes. Isoagglutinins for red cells were not detected in any of the strains of rats tested, but rabbit xenoagglutinins to rat and sheep red cells were found. Reagent Ag‐C antisera were used to type some previously unreported strains of inbred rats.