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EFFECT OF CHEMOTHERAPEUTICS ON LYMPHOCYTE HL‐A ANTIGENS IN VITRO
Author(s) -
Mäjský A.,
Chudomel V.,
Jakoubková J.
Publication year - 1975
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1975.tb00519.x
Subject(s) - cytotoxic t cell , antigen , incubation , cyclophosphamide , lymphocyte , in vitro , cytotoxicity , immunology , biology , chemistry , biochemistry , chemotherapy , genetics
SUMMARY The effect of chemotherapeutics on the lymphocyte HL‐A antigens in vitro depends on the character of the preparation and on the individual variability of the lymphocytes. There is no general parallelism between the effects on the cytotoxic reactivity and absorption capacity. Sometimes both activities are suppressed, sometimes only the absorption capacity is reduced and the treated lymphocytes are polyreactive in the cytotoxic test. Alexan was ineffective. TS 160, cytembena and alkeran reduced the absorption capacity of the HL‐A antigens already after 2‐hr treatment of the lymphocytes, whereas in the cytotoxic test the lymphocytes were polyreactive owing to their remarkable damage caused by these substances. Cyclophosphamide was able to reduce the absorption capacity of HL‐A antigens only after 20 hr at 20°C or after 2 hr of exposure to a higher dose of the substance. After the 2‐hr treatment the lymphocytes showed various types of effect in the cytotoxic test—sometimes their reactivity remained unaltered, sometimes it disappeared; in other cases they became polyreactive and after 20‐hr incubation the cytotoxic reactivity was lost. The effect of methotrexate on the absorption capacity of HL‐A antigens was the same as that of cyclophosphamide, whereas by contrast, the cytotoxic reactivity remained unaltered after 2‐hr incubation; after longer periods of time the lymphocytes become polyreactive. Vincristin suppressed the absorption capacity only after the 20‐hr exposure and thus treated lymphocytes were polyreactive. After the 2‐hr incubation the effect was the same as that for cyclophosphamide.